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ASK1 goes on to bind to TRAF2, which in turn activates downstream-signaling cascades, including the MKK3/4/6p38 and MKK4/7JNK signaling pathways 36,37 (Figure 4)

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Augmenting wheat fiber consumption requires several months but ultimately leads to enhanced production of short-chain fatty acids (SCFA) and release of glucagon-like peptide-1 (GLP-1)

GLP-1 Fc-fusion was eluted with a gradient (15 column volumes) 50 mM sodium acetate, pH 3.65

Therefore, we pretreated cell lysates with 8 mM CaCl 2 (see methods for details) to clear out the ER and mitochondria contaminants and repeated the Lyso-IP experiment (Figure 2D, fourth lane)